Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 17-42
... Escherichia coli . Gene 40 : 183 . Amann , E. , J. Brosius , and M. Ptashne . 1983. Vectors bearing a hybrid trp - lac promoter useful for regulated expres- sion of cloned genes in Escherichia coli . Gene 25 : 167 . Buell , G. , M.-F ...
... Escherichia coli . Gene 40 : 183 . Amann , E. , J. Brosius , and M. Ptashne . 1983. Vectors bearing a hybrid trp - lac promoter useful for regulated expres- sion of cloned genes in Escherichia coli . Gene 25 : 167 . Buell , G. , M.-F ...
Page 17-43
... Escherichia coli . Nucleic Acids Res . 9 : 5671 . Laemmli , U.K. 1970. Cleavage of struc- tural proteins during the assembly of the head of bacteriophage T4 . Nature 227 : 680 . Marquis , D.M. , J.M. Smolec , and D.H. Katz . 1986. Use ...
... Escherichia coli . Nucleic Acids Res . 9 : 5671 . Laemmli , U.K. 1970. Cleavage of struc- tural proteins during the assembly of the head of bacteriophage T4 . Nature 227 : 680 . Marquis , D.M. , J.M. Smolec , and D.H. Katz . 1986. Use ...
Page R-1
... E. coli DH5a compe- tent cells . Bethesda Res . Lab . Focus 8 ( 2 ) : 9 . Bolivar , F. and K. Backman . 1979. Plas- mids of Escherichia coli as cloning vec- tors . Methods Enzymol . 68 : 245 . Bolivar , F. , R.L. Rodriguez , P.J. Greene ...
... E. coli DH5a compe- tent cells . Bethesda Res . Lab . Focus 8 ( 2 ) : 9 . Bolivar , F. and K. Backman . 1979. Plas- mids of Escherichia coli as cloning vec- tors . Methods Enzymol . 68 : 245 . Bolivar , F. , R.L. Rodriguez , P.J. Greene ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml