Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 1634
... Incubate the mixture for 20-30 minutes at room temperature , during which time a fine precipitate should form . At the end of the incubation , pipette the mixture up and down once to resuspend the precipitate . A commonly used ...
... Incubate the mixture for 20-30 minutes at room temperature , during which time a fine precipitate should form . At the end of the incubation , pipette the mixture up and down once to resuspend the precipitate . A commonly used ...
Page 1649
... Incubate the suspension for 5 minutes at 4 ° C . Lysozyme will not work efficiently if the pH of the solution is less than 8.0 . 4. Add 1 ml of ice - cold 0.25 m EDTA ( pH 8.0 ) . Incubate on ice for 5 minutes . • 5. Slowly add 1 ml of ...
... Incubate the suspension for 5 minutes at 4 ° C . Lysozyme will not work efficiently if the pH of the solution is less than 8.0 . 4. Add 1 ml of ice - cold 0.25 m EDTA ( pH 8.0 ) . Incubate on ice for 5 minutes . • 5. Slowly add 1 ml of ...
Page 17-19
... Incubate the mixture for 5 minutes at 70 ° C to denature the DNAs . c . Slowly cool to 18 ° C . d . Add 10 μl of 2 × elongation mix and 2 μl of the Klenow fragment of E. coli DNA polymerase I. Incubate for 30 minutes at room temperature ...
... Incubate the mixture for 5 minutes at 70 ° C to denature the DNAs . c . Slowly cool to 18 ° C . d . Add 10 μl of 2 × elongation mix and 2 μl of the Klenow fragment of E. coli DNA polymerase I. Incubate for 30 minutes at room temperature ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml