Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 11
Page C-7
... OH i OH -OCH2 H H H OH OH cytosine Lof Lofo HO- OH OH -O - P - OCH2 OH cytosine H H 483.2 271 9.0 0.97 H H OH OH cytosine HO -O- 2 ' - Deoxycytidine O - P - OCH2 5 ' - triphosphate ( dCTP ) OH OH OH H H I- 467.2 272 9.1 0.98 H H OH H C ...
... OH i OH -OCH2 H H H OH OH cytosine Lof Lofo HO- OH OH -O - P - OCH2 OH cytosine H H 483.2 271 9.0 0.97 H H OH OH cytosine HO -O- 2 ' - Deoxycytidine O - P - OCH2 5 ' - triphosphate ( dCTP ) OH OH OH H H I- 467.2 272 9.1 0.98 H H OH H C ...
Page C-8
... OH N 151.1 276 8.15 1.04 H2N N H OH Guanosine Guanosine 5 ' - phosphate ( 5 ' - GMP ) H2N HOCH2 0.67 N 283.2 253 13.6 H H H OH OH HO - P - OCH2 OH H guanine I- H OH OH H 363.2 252 13.7 0.66 ( 5 ' - GTP ) guanine H H OH.
... OH N 151.1 276 8.15 1.04 H2N N H OH Guanosine Guanosine 5 ' - phosphate ( 5 ' - GMP ) H2N HOCH2 0.67 N 283.2 253 13.6 H H H OH OH HO - P - OCH2 OH H guanine I- H OH OH H 363.2 252 13.7 0.66 ( 5 ' - GTP ) guanine H H OH.
Page C-9
... OH OH guanine HO - P ・ O ・ O - P - OCH2 523.2 253 13.7 0.66 H H OH OH guanine 2 ' - Deoxyguanosine 5 ' - triphosphate OH OH OH H H ( dGTP ) I- 507.2 253 13.7 0.66 H H OH H TABLE C.6 Thymine and Related Compounds Thymine HO N OH.
... OH OH guanine HO - P ・ O ・ O - P - OCH2 523.2 253 13.7 0.66 H H OH OH guanine 2 ' - Deoxyguanosine 5 ' - triphosphate OH OH OH H H ( dGTP ) I- 507.2 253 13.7 0.66 H H OH H TABLE C.6 Thymine and Related Compounds Thymine HO N OH.
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Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml