Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 18-45
... buffer best suited to a particular antigen - antibody combination should be determined in pilot experiments . In increasing order of stringency , these are : • NET - gel ... SDS ( final concentration 0.1 % ) . The third wash is carried out ...
... buffer best suited to a particular antigen - antibody combination should be determined in pilot experiments . In increasing order of stringency , these are : • NET - gel ... SDS ( final concentration 0.1 % ) . The third wash is carried out ...
Page 18-53
... SDS gel - loading buffer to denature the proteins . 1 × SDS gel - loading buffer 50 mM Tris . Cl ( pH 6.8 ) 100 mм dithiothreitol 2 % SDS ( electrophoresis grade ) 0.1 % bromophenol blue 10 % glycerol 1 × SDS gel - loading buffer ...
... SDS gel - loading buffer to denature the proteins . 1 × SDS gel - loading buffer 50 mM Tris . Cl ( pH 6.8 ) 100 mм dithiothreitol 2 % SDS ( electrophoresis grade ) 0.1 % bromophenol blue 10 % glycerol 1 × SDS gel - loading buffer ...
Page 18-63
... SDS gel - loading buffer . 2 × SDS gel - loading buffer • 100 mM Tris Cl ( pH 6.8 ) 200 mм dithiothreitol 4 % SDS ( electrophoresis grade ) 0.2 % bromophenol blue 20 % glycerol 2 × SDS gel - loading buffer lacking dithiothreitol can be ...
... SDS gel - loading buffer . 2 × SDS gel - loading buffer • 100 mM Tris Cl ( pH 6.8 ) 200 mм dithiothreitol 4 % SDS ( electrophoresis grade ) 0.2 % bromophenol blue 20 % glycerol 2 × SDS gel - loading buffer lacking dithiothreitol can be ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml