Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 18-49
... Tris buffers for the preparation of resolving and stacking gels : It is essen- tial that these buffers be prepared with Tris base . After the Tris base has been dissolved in deionized water , the pH of the solution should be adjusted ...
... Tris buffers for the preparation of resolving and stacking gels : It is essen- tial that these buffers be prepared with Tris base . After the Tris base has been dissolved in deionized water , the pH of the solution should be adjusted ...
Page 18-52
... Tris - glycine SDS - Polyacrylamide Gel Electrophoresis Component volumes ( ml ) per gel mold volume of Solution components 5 ml 10 ml 15 ml 20 ml 25 ml 30 ml 40 ml 50 ml 6 % H2O 2.6 5.3 7.9 10.6 13.2 15.9 21.2 26.5 30 % acrylamide mix ...
... Tris - glycine SDS - Polyacrylamide Gel Electrophoresis Component volumes ( ml ) per gel mold volume of Solution components 5 ml 10 ml 15 ml 20 ml 25 ml 30 ml 40 ml 50 ml 6 % H2O 2.6 5.3 7.9 10.6 13.2 15.9 21.2 26.5 30 % acrylamide mix ...
Page B-23
... Tris - acetate 0.001 M EDTA Tris - phosphate ( TPE ) Tris - borate " ( TBE ) 1 × : 0.09 M Tris - phosphate 0.002 M EDTA 0.5X : 0.045 M Tris - borate 0.001 M EDTA Alkalineb Tris - glycine 1x : 50 mn NaOH 1 mM EDTA 1x : 25 mM Tris 250 mM ...
... Tris - acetate 0.001 M EDTA Tris - phosphate ( TPE ) Tris - borate " ( TBE ) 1 × : 0.09 M Tris - phosphate 0.002 M EDTA 0.5X : 0.045 M Tris - borate 0.001 M EDTA Alkalineb Tris - glycine 1x : 50 mn NaOH 1 mM EDTA 1x : 25 mM Tris 250 mM ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml