Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 1657
... activity from activities endogenous to mammalian cells . The following considerations are important when selecting a reporter gene ( Gorman et al . 1982a ) : • The enzymatic activity encoded by the prokaryotic gene must be readily ...
... activity from activities endogenous to mammalian cells . The following considerations are important when selecting a reporter gene ( Gorman et al . 1982a ) : • The enzymatic activity encoded by the prokaryotic gene must be readily ...
Page 1658
... activity that can be assayed in the same cell extracts prepared for measurement of CAT activity . An enzyme frequently used for this purpose is E. coli ẞ - galactosidase , which is expressed in transfected mammalian cells from a ...
... activity that can be assayed in the same cell extracts prepared for measurement of CAT activity . An enzyme frequently used for this purpose is E. coli ẞ - galactosidase , which is expressed in transfected mammalian cells from a ...
Page 18-2
... activity or by employing assays that are independent of such activity . In a few cases , the protein of interest carries an enzymatic or other biological activity ( e.g. , the ability to bind a specific ligand ) that can be assayed in ...
... activity or by employing assays that are independent of such activity . In a few cases , the protein of interest carries an enzymatic or other biological activity ( e.g. , the ability to bind a specific ligand ) that can be assayed in ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml