Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 1639
... aliquots at -20 ° C . 2. Twenty - four hours before transfection , harvest exponentially growing cells by trypsinization and replate aliquots of 5 x 105 cells onto 90 - mm tissue culture dishes . Add 10 ml of complete growth medium ...
... aliquots at -20 ° C . 2. Twenty - four hours before transfection , harvest exponentially growing cells by trypsinization and replate aliquots of 5 x 105 cells onto 90 - mm tissue culture dishes . Add 10 ml of complete growth medium ...
Page B-11
... aliquots . Extinction Coefficient ( e ) ( M1 cm ̄1 ) -1 Wavelength Base ( nm ) A 259 1.54 × 101 G 253 1.37 x 10 * C 271 9.10 × 103 T 260 7.40 × 103 For a cuvette with a path length of 1 cm , absorbance = € M . 100 mм stock solutions of ...
... aliquots . Extinction Coefficient ( e ) ( M1 cm ̄1 ) -1 Wavelength Base ( nm ) A 259 1.54 × 101 G 253 1.37 x 10 * C 271 9.10 × 103 T 260 7.40 × 103 For a cuvette with a path length of 1 cm , absorbance = € M . 100 mм stock solutions of ...
Page B-17
... aliquots and store at -20 ° C . Discard each aliquot after use . RNAase That Is Free of DNAase Dissolve pancreatic RNAase ( RNAase A ) at a concentration of 10 mg / ml in 0.01 M sodium acetate ( pH 5.2 ) . Heat to 100 ° C for 15 minutes ...
... aliquots and store at -20 ° C . Discard each aliquot after use . RNAase That Is Free of DNAase Dissolve pancreatic RNAase ( RNAase A ) at a concentration of 10 mg / ml in 0.01 M sodium acetate ( pH 5.2 ) . Heat to 100 ° C for 15 minutes ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml