Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 65
Page 17-31
... allow secretion of a given foreign protein . Even then , processing may not occur in the correct position and protein levels may be low . One system that may allow secretion and processing takes advantage of the alkaline phosphatase ...
... allow secretion of a given foreign protein . Even then , processing may not occur in the correct position and protein levels may be low . One system that may allow secretion and processing takes advantage of the alkaline phosphatase ...
Page A-4
... allow about 30-35 ml of medium per 90 - mm plate . To remove bubbles from medium in the plate , flame the surface of ... allows bacterial colonies or bacteriophage plaques to spread across the surfaces of the plates and increases the ...
... allow about 30-35 ml of medium per 90 - mm plate . To remove bubbles from medium in the plate , flame the surface of ... allows bacterial colonies or bacteriophage plaques to spread across the surfaces of the plates and increases the ...
Page E-21
... allow the emitted ẞ particles to interact productively with silver halide crystals in the emulsion , it is not enough to allow the particles to pass through barriers ( e.g. , Saran Wrap ) that might be placed between the film and the ...
... allow the emitted ẞ particles to interact productively with silver halide crystals in the emulsion , it is not enough to allow the particles to pass through barriers ( e.g. , Saran Wrap ) that might be placed between the film and the ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml