Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 18-4
... ANTIGEN Aggregated protein is a much better immunogen than soluble , dispersed protein . Partial denaturation may help to increase immunogenicity but may yield antisera that fail to react with native ... ANTIGEN 18 4 AMOUNT OF ANTIGEN 18 4.
... ANTIGEN Aggregated protein is a much better immunogen than soluble , dispersed protein . Partial denaturation may help to increase immunogenicity but may yield antisera that fail to react with native ... ANTIGEN 18 4 AMOUNT OF ANTIGEN 18 4.
Page 18-5
... antigen . The length of these intervals varies from antigen to antigen and cannot be predicted in advance . The best procedure is to boost the animal when the titer of circulating antibody begins to drop . The amount of antigen used for ...
... antigen . The length of these intervals varies from antigen to antigen and cannot be predicted in advance . The best procedure is to boost the animal when the titer of circulating antibody begins to drop . The amount of antigen used for ...
Page 18-21
... antigen- antibody complexes bound to a solid support varies from antigen to antigen . Typically , however , a saturating amount of protein antigen attached to an immobilized first antibody might be expected to bind approximately 2 × 101 ...
... antigen- antibody complexes bound to a solid support varies from antigen to antigen . Typically , however , a saturating amount of protein antigen attached to an immobilized first antibody might be expected to bind approximately 2 × 101 ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml