Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 18-4
... antisera that fail to react with native protein . Severely denatured proteins ( e.g. , proteins that have been extensively boiled , reduced , and alkylated ) often yield antisera that react exclusively or preferentially with denatured ...
... antisera that fail to react with native protein . Severely denatured proteins ( e.g. , proteins that have been extensively boiled , reduced , and alkylated ) often yield antisera that react exclusively or preferentially with denatured ...
Page 18-10
... antiserum to a fresh tube , and then centrifuge the clot at 1500g for 10 minutes at room temperature . Combine the supernatant with the previously removed antiserum , discard the clot , and store the antiserum . Antisera may be stored ...
... antiserum to a fresh tube , and then centrifuge the clot at 1500g for 10 minutes at room temperature . Combine the supernatant with the previously removed antiserum , discard the clot , and store the antiserum . Antisera may be stored ...
Page 18-14
... antisera raised in experimental animals contain very high concentrations of immunoglobulin directed against the target antigen , such antisera also always contain antibodies directed against other antigens . In addition , the ...
... antisera raised in experimental animals contain very high concentrations of immunoglobulin directed against the target antigen , such antisera also always contain antibodies directed against other antigens . In addition , the ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml