Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 1658
... assay . The CAT activity is then normalized to the ẞ - galactosidase activity . Alternatively , the ẞ - galactosidase activity in a constant volume of extract is measured and the CAT assay is then carried out with amounts of extract ...
... assay . The CAT activity is then normalized to the ẞ - galactosidase activity . Alternatively , the ẞ - galactosidase activity in a constant volume of extract is measured and the CAT assay is then carried out with amounts of extract ...
Page 1666
... assay ) . The extracts that are to be used for assay of CAT activity should be heated to 65 ° C for 10 minutes after the B - galactosidase assays are completed . ii . It is essential to include positive and negative controls of the ...
... assay ) . The extracts that are to be used for assay of CAT activity should be heated to 65 ° C for 10 minutes after the B - galactosidase assays are completed . ii . It is essential to include positive and negative controls of the ...
Page 18-19
... assay can be extremely sensitive but requires that target protein is available ( prefer- ably in a pure form ) to serve both as a competitor and as a standard . • Immobilized antigen RIAs : In this method , unlabeled antigen is attached ...
... assay can be extremely sensitive but requires that target protein is available ( prefer- ably in a pure form ) to serve both as a competitor and as a standard . • Immobilized antigen RIAs : In this method , unlabeled antigen is attached ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml