Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 17
Page A-2
... autoclaving for 20 minutes at 15 lb / sq . in . on liquid cycle . 4 2 Allow the solution to cool to 60 ° C or less , and then add 100 ml of a sterile solution of 0.17 м KH2PO4 , 0.72 м K2HPO4 . ( This solution is made by dissolving 2.31 ...
... autoclaving for 20 minutes at 15 lb / sq . in . on liquid cycle . 4 2 Allow the solution to cool to 60 ° C or less , and then add 100 ml of a sterile solution of 0.17 м KH2PO4 , 0.72 м K2HPO4 . ( This solution is made by dissolving 2.31 ...
Page A-3
... autoclaving for 15 minutes at 15 lb / sq . in . on liquid cycle . The MgSO , and CaCl , solutions should be prepared separately , sterilized by autoclaving , and added after diluting the 5 × M9 salts to 1 liter with sterile deionized ...
... autoclaving for 15 minutes at 15 lb / sq . in . on liquid cycle . The MgSO , and CaCl , solutions should be prepared separately , sterilized by autoclaving , and added after diluting the 5 × M9 salts to 1 liter with sterile deionized ...
Page A-7
... autoclaving the solution for 15 minutes at 15 lb / sq . in . on liquid cycle . Per liter : • 1 м Tris Cl ( pH 7.5 ) 50 ml MgSO4 · 7H2O 2 g H2O to 1 liter Sterilize the buffer by autoclaving for 20 minutes at 15 lb / sq . in . on liquid ...
... autoclaving the solution for 15 minutes at 15 lb / sq . in . on liquid cycle . Per liter : • 1 м Tris Cl ( pH 7.5 ) 50 ml MgSO4 · 7H2O 2 g H2O to 1 liter Sterilize the buffer by autoclaving for 20 minutes at 15 lb / sq . in . on liquid ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml