Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 1649
... bacteria containing the plasmid of interest . When the bacteria have grown to mid - log phase ( ~ 2 × 108 bacteria / ml ) , chloramphenicol should be added from a stock solution ( 34 mg / ml in absolute ethanol and sterilized by passage ...
... bacteria containing the plasmid of interest . When the bacteria have grown to mid - log phase ( ~ 2 × 108 bacteria / ml ) , chloramphenicol should be added from a stock solution ( 34 mg / ml in absolute ethanol and sterilized by passage ...
Page 18-29
... BACTERIA Unlike most vectors used in mammalian cells , vectors used to express foreign proteins in yeasts and bacteria usually contain powerful , inducible promot- ers . The protocols used to radiolabel proteins expressed from different ...
... BACTERIA Unlike most vectors used in mammalian cells , vectors used to express foreign proteins in yeasts and bacteria usually contain powerful , inducible promot- ers . The protocols used to radiolabel proteins expressed from different ...
Page A-5
... bacteria , pick a single , well - isolated colony with a sterile inoculat- ing needle and stab the needle several ... Bacterial Media , Antibiotics , and Bacterial Strains A.5.
... bacteria , pick a single , well - isolated colony with a sterile inoculat- ing needle and stab the needle several ... Bacterial Media , Antibiotics , and Bacterial Strains A.5.
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml