Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 1578
... Bacteriophage SP6 and Bacteriophages T7 and T3 RNA Polymerases 5.58 LIGASES , KINASES , AND PHOSPHATASES 5.61 Bacteriophage T4 DNA Ligase 5.62 E. coli DNA Ligase 5.64 Bacteriophage T4 RNA Ligase 5.66 Bacteriophage T4 Polynucleotide ...
... Bacteriophage SP6 and Bacteriophages T7 and T3 RNA Polymerases 5.58 LIGASES , KINASES , AND PHOSPHATASES 5.61 Bacteriophage T4 DNA Ligase 5.62 E. coli DNA Ligase 5.64 Bacteriophage T4 RNA Ligase 5.66 Bacteriophage T4 Polynucleotide ...
Page F-4
... bacteriophage enzyme is generally preferred for removal of protruding 3 ' termini because its 3 ' exonuclease activity is more than 200 - fold more active than that of the Klenow enzyme . Bacteriophage T4 DNA polymerase has a pH optimum ...
... bacteriophage enzyme is generally preferred for removal of protruding 3 ' termini because its 3 ' exonuclease activity is more than 200 - fold more active than that of the Klenow enzyme . Bacteriophage T4 DNA polymerase has a pH optimum ...
Page F-9
... bacteriophage T4 DNA ligase ( 1-2 Weiss units ) Incubate the reaction for 6-16 hours at 16 ° C . 10x Blunt - end ligation buffer 0.66 M Tris Cl ( pH 7.6 ) 50 mM MgCl2 50 mm dithiothreitol 1 mg / ml bovine serum albumin ( Fraction V ...
... bacteriophage T4 DNA ligase ( 1-2 Weiss units ) Incubate the reaction for 6-16 hours at 16 ° C . 10x Blunt - end ligation buffer 0.66 M Tris Cl ( pH 7.6 ) 50 mM MgCl2 50 mm dithiothreitol 1 mg / ml bovine serum albumin ( Fraction V ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml