Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 27
Page 18-49
... base . After the Tris base has been dissolved in deionized water , the pH of the solution should be adjusted with HCl as described in Appendix B. If Tris Cl or Trizma is used to prepare buffers , the concentration of salt will be too ...
... base . After the Tris base has been dissolved in deionized water , the pH of the solution should be adjusted with HCl as described in Appendix B. If Tris Cl or Trizma is used to prepare buffers , the concentration of salt will be too ...
Page B-23
... base 57.1 ml glacial acetic acid 100 ml 0.5 M EDTA ( pH 8.0 ) 10x : 108 g Tris base 15.5 ml 85 % phosphoric acid ( 1.679 g / ml ) 40 ml 0.5 M EDTA ( pH 8.0 ) 5x : 54 g Tris base 27.5 g boric acid 20 ml 0.5 M EDTA ( pH 8.0 ) 1x : 5 ml 10 ...
... base 57.1 ml glacial acetic acid 100 ml 0.5 M EDTA ( pH 8.0 ) 10x : 108 g Tris base 15.5 ml 85 % phosphoric acid ( 1.679 g / ml ) 40 ml 0.5 M EDTA ( pH 8.0 ) 5x : 54 g Tris base 27.5 g boric acid 20 ml 0.5 M EDTA ( pH 8.0 ) 1x : 5 ml 10 ...
Page C-13
... base = adenine 2 ' , 3 ' - Dideoxycytidine 5 ' - triphosphate base = cytosine ( ddCTP ) 2 ' , 3 ' - Dideoxyguanosine 5 ' - triphosphate base = guanine ( ddGTP ) 2 ' , 3 ' - Dideoxythymidine 5 ' - triphosphate ( ddTTP ) base = thymine ...
... base = adenine 2 ' , 3 ' - Dideoxycytidine 5 ' - triphosphate base = cytosine ( ddCTP ) 2 ' , 3 ' - Dideoxyguanosine 5 ' - triphosphate base = guanine ( ddGTP ) 2 ' , 3 ' - Dideoxythymidine 5 ' - triphosphate ( ddTTP ) base = thymine ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml