Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 17-17
... BINDING SITES In addition to the bacterial promoter , the second major factor required to express cloned DNA in E. coli is an efficient ribosome - binding site . In E. coli , the ribosome - binding site includes an initiation codon ...
... BINDING SITES In addition to the bacterial promoter , the second major factor required to express cloned DNA in E. coli is an efficient ribosome - binding site . In E. coli , the ribosome - binding site includes an initiation codon ...
Page 18-11
... binding sites for protein A. Because protein A itself has four potential sites for binding to IgG ( Sjödahl 1977 ) , it is possible to form multimeric complexes of the two types of protein . Not all immunoglobulins bind to protein A ...
... binding sites for protein A. Because protein A itself has four potential sites for binding to IgG ( Sjödahl 1977 ) , it is possible to form multimeric complexes of the two types of protein . Not all immunoglobulins bind to protein A ...
Page 18-69
... bind to the nitrocellulose filter , so can proteins in the immunological reagents used for probing . The sensitivity of western blotting depends on reducing this background of nonspecific binding by blocking potential binding sites with ...
... bind to the nitrocellulose filter , so can proteins in the immunological reagents used for probing . The sensitivity of western blotting depends on reducing this background of nonspecific binding by blocking potential binding sites with ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml