Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 1609
... cells in a given culture , becomes stably maintained in an even smaller fraction . In a very few cases - for example , when the cells are transformed by an oncogene stably transfected cells can be identified because they express an ...
... cells in a given culture , becomes stably maintained in an even smaller fraction . In a very few cases - for example , when the cells are transformed by an oncogene stably transfected cells can be identified because they express an ...
Page 1635
... cells to the toxic effect of the drug . The optimal concentration of chloroquine for the particular cell type that is used should therefore be determined in preliminary experiments . However , most cell types respond well to treatment ...
... cells to the toxic effect of the drug . The optimal concentration of chloroquine for the particular cell type that is used should therefore be determined in preliminary experiments . However , most cell types respond well to treatment ...
Page 1638
... cells by centrifugation at 800g for 5 minutes at 4 ° C . Discard the supernatant , and resuspend the cell pellet in 20 volumes of ice - cold phosphate ... Cells Calcium Phosphate-mediated Transfection of Cells Growing in Suspension 16 38.
... cells by centrifugation at 800g for 5 minutes at 4 ° C . Discard the supernatant , and resuspend the cell pellet in 20 volumes of ice - cold phosphate ... Cells Calcium Phosphate-mediated Transfection of Cells Growing in Suspension 16 38.
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml