Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 1661
A Laboratory Manual Joseph Sambrook, Tom Maniatis. The acetylated forms of chloramphenicol partition into the organic ( upper ) phase ; unacetylated chloramphenicol remains in the aqueous phase . 8. Using an automatic pipettor , transfer ...
A Laboratory Manual Joseph Sambrook, Tom Maniatis. The acetylated forms of chloramphenicol partition into the organic ( upper ) phase ; unacetylated chloramphenicol remains in the aqueous phase . 8. Using an automatic pipettor , transfer ...
Page 1663
... chloramphenicol cell extract 0.25 M Tris - Cl ( pH 7.8 ) solution containing 14C - labeled acetyl coenzyme A 20 με 30 με 30 μ . 20 μl 8 mM chloramphenicol ( M , = 321 ) is made by dissolving 2.57 mg of chloramphenicol in 1 ml of ethanol ...
... chloramphenicol cell extract 0.25 M Tris - Cl ( pH 7.8 ) solution containing 14C - labeled acetyl coenzyme A 20 με 30 με 30 μ . 20 μl 8 mM chloramphenicol ( M , = 321 ) is made by dissolving 2.57 mg of chloramphenicol in 1 ml of ethanol ...
Page 1664
... chloramphenicol into ethyl acetate . Rather , the reaction mixture is overlaid with water - immiscible scintillation fluid . Acetylated forms of chloramphenicol produced by the activity of CAT in the aqueous phase diffuse rapidly into ...
... chloramphenicol into ethyl acetate . Rather , the reaction mixture is overlaid with water - immiscible scintillation fluid . Acetylated forms of chloramphenicol produced by the activity of CAT in the aqueous phase diffuse rapidly into ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml