Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 1602
... cloned genes in a broad range of cell types from different species . These methods have been used to overproduce proteins for structural and biochemical studies ... CLONED GENES A 16.2 Expression of Cloned Genes in Cultured Mammalian Cells.
... cloned genes in a broad range of cell types from different species . These methods have been used to overproduce proteins for structural and biochemical studies ... CLONED GENES A 16.2 Expression of Cloned Genes in Cultured Mammalian Cells.
Page 1603
... CLONED GENES A few eukaryotic proteins have been expressed efficiently and inexpensively in prokaryotic hosts ( see Chapter 17 ) . However , many eukaryotic proteins synthesized in bacteria fold incorrectly or inefficiently and ...
... CLONED GENES A few eukaryotic proteins have been expressed efficiently and inexpensively in prokaryotic hosts ( see Chapter 17 ) . However , many eukaryotic proteins synthesized in bacteria fold incorrectly or inefficiently and ...
Page 17-11
... cloned gene that has an E. coli ribosome - binding site : 1. Digest pKC30 with HpaI . 2. Digest the cloned DNA with appropriate restriction enzymes at a position 5 ' of the initiation codon and at a site 3 ' of the terminus of the ...
... cloned gene that has an E. coli ribosome - binding site : 1. Digest pKC30 with HpaI . 2. Digest the cloned DNA with appropriate restriction enzymes at a position 5 ' of the initiation codon and at a site 3 ' of the terminus of the ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml