Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 1616
... codon of the natural gene are almost invariably replaced by a Shine - Dalgarno ribosome - binding sequence and an ATG codon that have been optimally spaced for efficient expression . With mammalian mRNAs , the number of nucleotides ...
... codon of the natural gene are almost invariably replaced by a Shine - Dalgarno ribosome - binding sequence and an ATG codon that have been optimally spaced for efficient expression . With mammalian mRNAs , the number of nucleotides ...
Page 17-22
... codon ( Panayotatos and Truong 1981 ; Shatzman et al . 1983 ) . This can be done as follows ( see Figure 17.7 , pages 17.20-17.21 ) : 1. Insert two unique restriction sites upstream of the coding sequence to be expressed by inserting ...
... codon ( Panayotatos and Truong 1981 ; Shatzman et al . 1983 ) . This can be done as follows ( see Figure 17.7 , pages 17.20-17.21 ) : 1. Insert two unique restriction sites upstream of the coding sequence to be expressed by inserting ...
Page D-1
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Appendix D : Codons and Amino Acids 2nd position of codon U C A G บ บ บ Phe UCU Ser UAU Tyr UGU Cys UUC Phe UCC Ser UAC Tyr UGC Cys U UUA Leu UCA Ser UAA Stop ( Ochre ) UGA Stop ( Umber ) ...
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Appendix D : Codons and Amino Acids 2nd position of codon U C A G บ บ บ Phe UCU Ser UAU Tyr UGU Cys UUC Phe UCC Ser UAC Tyr UGC Cys U UUA Leu UCA Ser UAA Stop ( Ochre ) UGA Stop ( Umber ) ...
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Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml