Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 96
Page 17-43
... coli . Gene 42 : 175 . Marston , F.A.O. 1987. The purification of eukaryotic polypeptides expressed in Escherichia coli . In DNA cloning : A practical approach ( ed . D.M. Glover ) , vol . 3 , p . 59. IRL Press , Oxford . Marston ...
... coli . Gene 42 : 175 . Marston , F.A.O. 1987. The purification of eukaryotic polypeptides expressed in Escherichia coli . In DNA cloning : A practical approach ( ed . D.M. Glover ) , vol . 3 , p . 59. IRL Press , Oxford . Marston ...
Page 1-20
... coli strain , 4.15 , A.13 JM105 , E. coli strain genotype and uses , A.10 use in propagation of bacteriophage M13 vectors , 4.14 , A.13 JM106 , E. coli strain , 4.15 , A.13 JM107 , E. coli strain genotype and uses , A.10 use in ...
... coli strain , 4.15 , A.13 JM105 , E. coli strain genotype and uses , A.10 use in propagation of bacteriophage M13 vectors , 4.14 , A.13 JM106 , E. coli strain , 4.15 , A.13 JM107 , E. coli strain genotype and uses , A.10 use in ...
Page 1-37
... coli strain , genotype and uses , 17.13 , A.11 recA , genetic marker in E. coli host strain , 2.57 , 3.6 , 4.13 RecA protein , characteristics and use , 5.88 rec ̄ strains of E. coli , 3.6 , 3.34 . See also E. coli , strain list recB ...
... coli strain , genotype and uses , 17.13 , A.11 recA , genetic marker in E. coli host strain , 2.57 , 3.6 , 4.13 RecA protein , characteristics and use , 5.88 rec ̄ strains of E. coli , 3.6 , 3.34 . See also E. coli , strain list recB ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml