Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 27
Page 18-12
... column , and then wash the column with 10 column volumes of 100 mm Tris Cl ( pH 8.0 ) followed by 10 volumes of 10 mM Tris Cl ( pH 8.0 ) . • 4. Add 1 column volume of 100 mm glycine ( pH 3.0 ) . Begin collecting 500 - μl fractions of the ...
... column , and then wash the column with 10 column volumes of 100 mm Tris Cl ( pH 8.0 ) followed by 10 volumes of 10 mM Tris Cl ( pH 8.0 ) . • 4. Add 1 column volume of 100 mm glycine ( pH 3.0 ) . Begin collecting 500 - μl fractions of the ...
Page 18-24
... column in a pasteur pipette that is plugged with sterile glass wool . Run at least 10 column volumes of PBS containing 1 % bovine serum albumin through the column . Then wash the column with 5 volumes of PBS lacking bovine serum albumin ...
... column in a pasteur pipette that is plugged with sterile glass wool . Run at least 10 column volumes of PBS containing 1 % bovine serum albumin through the column . Then wash the column with 5 volumes of PBS lacking bovine serum albumin ...
Page E-32
... column . Bio - Gel HTP has a capacity of 100-200 μg of native DNA per milliliter of bed volume . 4. Prepare the hydroxyapatite columns in disposable 3 - ml plastic syringes as follows : a . Remove the barrel from the syringe . b . Using ...
... column . Bio - Gel HTP has a capacity of 100-200 μg of native DNA per milliliter of bed volume . 4. Prepare the hydroxyapatite columns in disposable 3 - ml plastic syringes as follows : a . Remove the barrel from the syringe . b . Using ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml