Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 11
Page A-1
... deionized H2O , add : bacto - tryptone 10 g bacto - yeast extract 5 g 10 g NaCl Shake until the solutes have dissolved . Adjust the pH to 7.0 with 5 N NaOH ( ~ 0.2 ml ) . Adjust the volume of the solution to 1 liter with deionized H2O ...
... deionized H2O , add : bacto - tryptone 10 g bacto - yeast extract 5 g 10 g NaCl Shake until the solutes have dissolved . Adjust the pH to 7.0 with 5 N NaOH ( ~ 0.2 ml ) . Adjust the volume of the solution to 1 liter with deionized H2O ...
Page A-2
... deionized H2O . After the salts have dissolved , adjust the volume of the solution to 100 ml with deionized H2O and sterilize by autoclaving for 20 minutes at 15 lb / sq . in . on liquid cycle . ) SOB Medium Per liter : To 950 ml of ...
... deionized H2O . After the salts have dissolved , adjust the volume of the solution to 100 ml with deionized H2O and sterilize by autoclaving for 20 minutes at 15 lb / sq . in . on liquid cycle . ) SOB Medium Per liter : To 950 ml of ...
Page A-3
... deionized H2O , add : bacto - tryptone 16 g bacto - yeast extract 10 g NaCl 5 g Shake until the solutes have dissolved . Adjust the pH to 7.0 with 5 N NaOH . Adjust the volume of the solution to 1 liter with deionized H2O . Sterilize by ...
... deionized H2O , add : bacto - tryptone 16 g bacto - yeast extract 10 g NaCl 5 g Shake until the solutes have dissolved . Adjust the pH to 7.0 with 5 N NaOH . Adjust the volume of the solution to 1 liter with deionized H2O . Sterilize by ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml