Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 1638
... described on pages 16.33-16.34 , steps 1 and 3 . 2. While the precipitate is forming , collect the exponentially growing cells by centrifugation at 800g for 5 minutes at 4 ° C . Discard the supernatant , and resuspend the cell pellet in ...
... described on pages 16.33-16.34 , steps 1 and 3 . 2. While the precipitate is forming , collect the exponentially growing cells by centrifugation at 800g for 5 minutes at 4 ° C . Discard the supernatant , and resuspend the cell pellet in ...
Page 17-25
... described above ( see pages 17.18-17.24 ) . In most cases , a blunt - ended fragment will be inserted and this step will not be required . In a few cases , the gene fragment prepared as described on pages 17.18- 17.24 will contain a ...
... described above ( see pages 17.18-17.24 ) . In most cases , a blunt - ended fragment will be inserted and this step will not be required . In a few cases , the gene fragment prepared as described on pages 17.18- 17.24 will contain a ...
Page 18-80
... described on pages 18.44-18.46 , and detailed and standardized procedures for electrophoresis in SDS - poly- acrylamide gels are described on pages 18.47-18.54 . Notes i . For analysis by SDS - polyacrylamide gel electrophoresis ...
... described on pages 18.44-18.46 , and detailed and standardized procedures for electrophoresis in SDS - poly- acrylamide gels are described on pages 18.47-18.54 . Notes i . For analysis by SDS - polyacrylamide gel electrophoresis ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml