Molecular Cloning: A Laboratory Manual, Book 3 |
From inside the book
Results 1-3 of 26
Page 18-21
... dilution of antigen that will give half - maximal binding of radiolabeled antibody . In this case , it is essential that the dilutions span a wide range from background to maximal binding . For accurate quantitation of antigen , it is ...
... dilution of antigen that will give half - maximal binding of radiolabeled antibody . In this case , it is essential that the dilutions span a wide range from background to maximal binding . For accurate quantitation of antigen , it is ...
Page 18-23
... dilutions in blocking buffer of the antigen that is to be assayed . Add 50 μl of each dilution to each of two wells . Cover the plate and incubate it for 2 hours at room temperature in a humidified atmo- sphere or for 8 hours at 4 ° C ...
... dilutions in blocking buffer of the antigen that is to be assayed . Add 50 μl of each dilution to each of two wells . Cover the plate and incubate it for 2 hours at room temperature in a humidified atmo- sphere or for 8 hours at 4 ° C ...
Page B-23
... dilution of the concentrated stock ) for agarose gel electrophoresis . However , a working solution of 0.5 × provides more than enough buffering power , and almost all agarose gel electrophoresis is now carried out with a 1:10 dilution ...
... dilution of the concentrated stock ) for agarose gel electrophoresis . However , a working solution of 0.5 × provides more than enough buffering power , and almost all agarose gel electrophoresis is now carried out with a 1:10 dilution ...
Other editions - View all
Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml