Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 1642
... dish ( or 35 - mm dishes with 5 × 10 cells / dish ) . Add 5 ml ( or 3 ml for 35 - mm dish ) of complete growth medium , and incubate the cultures for 20-24 hours at 37 ° C in a humidified incubator in an atmosphere of 5-7 % CO2 . The ...
... dish ( or 35 - mm dishes with 5 × 10 cells / dish ) . Add 5 ml ( or 3 ml for 35 - mm dish ) of complete growth medium , and incubate the cultures for 20-24 hours at 37 ° C in a humidified incubator in an atmosphere of 5-7 % CO2 . The ...
Page 1643
... dish ) or 3 ml ( per 35 - mm dish ) of prewarmed ( 37 ° C ) medium supplemented with serum and chloroquine diphosphate ( 100 μм final concentration ) , and incubate the cultures for 3-5 hours at 37 ° C in a humidified incubator in an ...
... dish ) or 3 ml ( per 35 - mm dish ) of prewarmed ( 37 ° C ) medium supplemented with serum and chloroquine diphosphate ( 100 μм final concentration ) , and incubate the cultures for 3-5 hours at 37 ° C in a humidified incubator in an ...
Page 1645
... dish ( or 35 - mm dishes with 5 x 10 cells / dish ) . Add 5 ml ( or 3 ml for 35 - mm dish ) of complete growth medium , and incubate the cultures for 20-24 hours at 37 ° C in a humidified incubator in an atmosphere of 5-7 % CO2 . The ...
... dish ( or 35 - mm dishes with 5 x 10 cells / dish ) . Add 5 ml ( or 3 ml for 35 - mm dish ) of complete growth medium , and incubate the cultures for 20-24 hours at 37 ° C in a humidified incubator in an atmosphere of 5-7 % CO2 . The ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml