Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 22
Page 17-9
... dithiothreitol 2 % SDS ( electrophoresis grade ) 0.1 % bromophenol blue 10 % glycerol 1x SDS gel - loading buffer lacking dithiothreitol can be stored at room temperature . Dithiothreitol should then be added , just before the buffer is ...
... dithiothreitol 2 % SDS ( electrophoresis grade ) 0.1 % bromophenol blue 10 % glycerol 1x SDS gel - loading buffer lacking dithiothreitol can be stored at room temperature . Dithiothreitol should then be added , just before the buffer is ...
Page B-25
... dithiothreitol 4 % SDS ( electrophoresis grade ) 0.2 % bromophenol blue 20 % glycerol 2 × SDS gel - loading buffer lacking dithiothreitol can be stored at room temperature . Dithiothreitol should then be added , just before the buffer ...
... dithiothreitol 4 % SDS ( electrophoresis grade ) 0.2 % bromophenol blue 20 % glycerol 2 × SDS gel - loading buffer lacking dithiothreitol can be stored at room temperature . Dithiothreitol should then be added , just before the buffer ...
Page B-27
... dithiothreitol 500 μg / ml bovine serum albumin ( Fraction V ; Sigma ) ( optional ) 10 × Blunt - end ligation buffer • 0.66 M Tris Cl ( pH 7.6 ) 50 mM MgCl2 50 mm dithiothreitol 1 mg / ml bovine serum albumin ( Fraction V ; Sigma ) ...
... dithiothreitol 500 μg / ml bovine serum albumin ( Fraction V ; Sigma ) ( optional ) 10 × Blunt - end ligation buffer • 0.66 M Tris Cl ( pH 7.6 ) 50 mM MgCl2 50 mm dithiothreitol 1 mg / ml bovine serum albumin ( Fraction V ; Sigma ) ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml