Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 1646
... efficiency . In these cases , it is best to transfect large monolayers of cells ( 90 - mm dishes ) and then to ... efficiency of stable transformation , which can vary over several orders of magnitude ( see , e.g. , Spandidos and Wilkie ...
... efficiency . In these cases , it is best to transfect large monolayers of cells ( 90 - mm dishes ) and then to ... efficiency of stable transformation , which can vary over several orders of magnitude ( see , e.g. , Spandidos and Wilkie ...
Page 1654
... efficiency of transfection has varied widely in reports from different laboratories . Recently , two groups have examined the parame- ters that affect transfection efficiencies and have shown that different condi- tions must be used for ...
... efficiency of transfection has varied widely in reports from different laboratories . Recently , two groups have examined the parame- ters that affect transfection efficiencies and have shown that different condi- tions must be used for ...
Page 1673
... efficiency COS cell expression system . Proc . Natl . Acad . Sci . 84 : 8573 . Bacchetti , S. and F.L. Graham . 1977 . Transfer of the gene for thymidine ki- nase to thymidine kinase - deficient human cells by purified herpes simplex ...
... efficiency COS cell expression system . Proc . Natl . Acad . Sci . 84 : 8573 . Bacchetti , S. and F.L. Graham . 1977 . Transfer of the gene for thymidine ki- nase to thymidine kinase - deficient human cells by purified herpes simplex ...
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Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml