Molecular Cloning: A Laboratory Manual, Book 3 |
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Page B-23
... ( electrophoresis grade ) ( pH 8.3 ) 50 ml 10 % SDS ( electrophoresis grade ) " A precipitate forms when concentrated solutions of TBE are stored for long periods of time . To avoid problems , store the 5 × solution in glass bottles at ...
... ( electrophoresis grade ) ( pH 8.3 ) 50 ml 10 % SDS ( electrophoresis grade ) " A precipitate forms when concentrated solutions of TBE are stored for long periods of time . To avoid problems , store the 5 × solution in glass bottles at ...
Page 1-1
... electrophoresis alkaline analysis of first- and second - strand cDNA synthesis , 8.5 analysis of single - stranded DNA , 6.20-6.21 bromocresol green as tracking dye in electrophoresis , 6.12 , 6.21 gel - loading buffer , 6.12 , B.24 ...
... electrophoresis alkaline analysis of first- and second - strand cDNA synthesis , 8.5 analysis of single - stranded DNA , 6.20-6.21 bromocresol green as tracking dye in electrophoresis , 6.12 , 6.21 gel - loading buffer , 6.12 , B.24 ...
Page 1-16
... electrophoresis FIGE ( field - inversion gel electrophoresis ) . See Pulsed - field gel electrophoresis Filamentous bacteriophages . See also M13 bacteriophage ; Phagemids as cloning vectors , 4.2 , 4.7 problems encountered in use ...
... electrophoresis FIGE ( field - inversion gel electrophoresis ) . See Pulsed - field gel electrophoresis Filamentous bacteriophages . See also M13 bacteriophage ; Phagemids as cloning vectors , 4.2 , 4.7 problems encountered in use ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml