Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 1610
... encoding aminoglycoside 3 ' phosphotransferase ( APH ) . Two distinct APH enzymes , encoded by the bacterial transposons Tn5 and Tn601 , confer resistance to aminoglycoside antibiotics such as kanamycin , neomycin , and geneticin ...
... encoding aminoglycoside 3 ' phosphotransferase ( APH ) . Two distinct APH enzymes , encoded by the bacterial transposons Tn5 and Tn601 , confer resistance to aminoglycoside antibiotics such as kanamycin , neomycin , and geneticin ...
Page 1615
... encoding aspartate transcarbamylase ( pyrB ) , when ex- pressed in CHO cells deficient in aspartate transcarbamylase , is also amplified by PALA selection ( Ruiz and Wahl 1986 ) . • Adenosine deaminase . Adenosine deaminase ( ADA ) is ...
... encoding aspartate transcarbamylase ( pyrB ) , when ex- pressed in CHO cells deficient in aspartate transcarbamylase , is also amplified by PALA selection ( Ruiz and Wahl 1986 ) . • Adenosine deaminase . Adenosine deaminase ( ADA ) is ...
Page 17-30
... encoding the factor X recognition site . a a 3. Clone a BamHI fragment encoding the factor X , site and the foreign gene into the BamHI site of pLcII , a plasmid encoding the bacteriophage › PL promoter that directs transcription of ...
... encoding the factor X recognition site . a a 3. Clone a BamHI fragment encoding the factor X , site and the foreign gene into the BamHI site of pLcII , a plasmid encoding the bacteriophage › PL promoter that directs transcription of ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml