Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 1572
... Ethidium Bromide Gradients CONTINUOUS GRADIENTS 1.42 DISCONTINUOUS GRADIENTS 1.44 1.42 Removal of Ethidium Bromide from DNAs Purified by Equilibrium Centrifugation in CsCl - Ethidium Bromide Gradients 1.46 METHOD 1 : EXTRACTION WITH ...
... Ethidium Bromide Gradients CONTINUOUS GRADIENTS 1.42 DISCONTINUOUS GRADIENTS 1.44 1.42 Removal of Ethidium Bromide from DNAs Purified by Equilibrium Centrifugation in CsCl - Ethidium Bromide Gradients 1.46 METHOD 1 : EXTRACTION WITH ...
Page E-8
A Laboratory Manual Joseph Sambrook, Tom Maniatis. DECONTAMINATION OF ETHIDIUM BROMIDE SOLUTIONS Caution : Ethidium bromide is a powerful mutagen and is moderately toxic . Gloves should be worn when working with solutions that contain ...
A Laboratory Manual Joseph Sambrook, Tom Maniatis. DECONTAMINATION OF ETHIDIUM BROMIDE SOLUTIONS Caution : Ethidium bromide is a powerful mutagen and is moderately toxic . Gloves should be worn when working with solutions that contain ...
Page E-9
... ethidium bromide with hypochlorite ( bleach ) is not recommended as a method of decontamination . Such treatment reduces the mutagenic activity of ethidium bromide in the Salmonella / microsome assay by about 1000 - fold , but it ...
... ethidium bromide with hypochlorite ( bleach ) is not recommended as a method of decontamination . Such treatment reduces the mutagenic activity of ethidium bromide in the Salmonella / microsome assay by about 1000 - fold , but it ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml