Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 1605
... eukaryotic transcription units that are expressed only in eukaryotic cells . The eukaryotic transcription unit consists of noncoding sequences and sequences coding for selectable markers . It is frequently assembled as a composite of ...
... eukaryotic transcription units that are expressed only in eukaryotic cells . The eukaryotic transcription unit consists of noncoding sequences and sequences coding for selectable markers . It is frequently assembled as a composite of ...
Page 1674
... eukaryotic cells . J. Mol . Biol . 150 : 1 . Colbère - Garapin , F. , S. Chousterman , F. Horodniceanu , P. Kourilsky , and A.-C. Garapin . 1979. Cloning of the active thymidine kinase gene of herpes sim- plex virus type 1 in ...
... eukaryotic cells . J. Mol . Biol . 150 : 1 . Colbère - Garapin , F. , S. Chousterman , F. Horodniceanu , P. Kourilsky , and A.-C. Garapin . 1979. Cloning of the active thymidine kinase gene of herpes sim- plex virus type 1 in ...
Page 1679
... eukaryotes . Trends Biochem . Sci . 14 : 105 . Proudfoot , N.J. and E. Whitelaw . 1988 . Termination and 3 ' end processing of eukaryotic RNA . In Transcription and splicing ( ed . B.D. Hames and D.M. Glover ) , p . 97. IRL Press ...
... eukaryotes . Trends Biochem . Sci . 14 : 105 . Proudfoot , N.J. and E. Whitelaw . 1988 . Termination and 3 ' end processing of eukaryotic RNA . In Transcription and splicing ( ed . B.D. Hames and D.M. Glover ) , p . 97. IRL Press ...
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Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml