Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 1604
... expression When choosing a mammalian expression vector , the following parameters should be taken into account : • The ... EXPRESSION VECTORS Mammalian expression vectors contain 16.4 Expression of Cloned Genes in Cultured Mammalian Cells.
... expression When choosing a mammalian expression vector , the following parameters should be taken into account : • The ... EXPRESSION VECTORS Mammalian expression vectors contain 16.4 Expression of Cloned Genes in Cultured Mammalian Cells.
Page 1617
A Laboratory Manual Joseph Sambrook, Tom Maniatis. VECTOR SYSTEMS A good way to establish rapidly the feasibility of expressing a cloned gene in mammalian cells is to use a transient expression system such as that provided by the simian ...
A Laboratory Manual Joseph Sambrook, Tom Maniatis. VECTOR SYSTEMS A good way to establish rapidly the feasibility of expressing a cloned gene in mammalian cells is to use a transient expression system such as that provided by the simian ...
Page 1-36
... vectors for preparing RNA , 10.29-10.31 polymerase chain reaction for synthesis , 14.5-14.9 primer extension of ... expression of prokaryotic genes in E. coli , 5.58 , 17.11-17.16 , 17.31- 17.33 in mammalian expression vectors , 16.5 ...
... vectors for preparing RNA , 10.29-10.31 polymerase chain reaction for synthesis , 14.5-14.9 primer extension of ... expression of prokaryotic genes in E. coli , 5.58 , 17.11-17.16 , 17.31- 17.33 in mammalian expression vectors , 16.5 ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml