Molecular Cloning: A Laboratory Manual, Book 3 |
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Page E-21
... film of the distribution of radioactive atoms on a two - dimensional surface . In molecular cloning , autoradiography is used for a variety of purposes , including the visualization of bands of radioactive nucleic acids in Southern and ...
... film of the distribution of radioactive atoms on a two - dimensional surface . In molecular cloning , autoradiography is used for a variety of purposes , including the visualization of bands of radioactive nucleic acids in Southern and ...
Page E-23
... film . To increase the efficiency with which these strong ẞ particles are detected , an intensifying screen may be placed behind the X - ray film . Radioactive particles that pass through the film hit the intensifying screen and cause ...
... film . To increase the efficiency with which these strong ẞ particles are detected , an intensifying screen may be placed behind the X - ray film . Radioactive particles that pass through the film hit the intensifying screen and cause ...
Page E-27
... film immediately and return the film holder and screens to the freezer as rapidly as possible . If condensation forms before you have time to apply a new film , allow the sample and screens to reach room temperature and wipe away all ...
... film immediately and return the film holder and screens to the freezer as rapidly as possible . If condensation forms before you have time to apply a new film , allow the sample and screens to reach room temperature and wipe away all ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml