Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 17-29
... protein . In particular , it may be important that the protein not have an amino - terminal methionine , which is ... foreign proteins are degraded by intracellular proteases . Two other expression systems may solve some of these problems .
... protein . In particular , it may be important that the protein not have an amino - terminal methionine , which is ... foreign proteins are degraded by intracellular proteases . Two other expression systems may solve some of these problems .
Page 17-31
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Expression of Secreted Foreign Proteins Secretion of the foreign protein is an alternative to intracellular expression . This is accomplished by fusing the coding sequence to DNA ...
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Expression of Secreted Foreign Proteins Secretion of the foreign protein is an alternative to intracellular expression . This is accomplished by fusing the coding sequence to DNA ...
Page 18-19
... foreign protein produced by transfected mammalian cell cultures . There are many different kinds of RIAS , which fall into four basic designs : Competition RIAS : In this method , the unlabeled target protein in the test sample competes ...
... foreign protein produced by transfected mammalian cell cultures . There are many different kinds of RIAS , which fall into four basic designs : Competition RIAS : In this method , the unlabeled target protein in the test sample competes ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml