Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 72
Page 17-18
... fragment extending from the second codon to a restriction site 3 ' of the cloned gene is made by one of the methods given below . SYNTHESIS OF DNA ENCODING THE AMINO TERMINUS 1. Synthesize a double - stranded DNA adapter extending from ...
... fragment extending from the second codon to a restriction site 3 ' of the cloned gene is made by one of the methods given below . SYNTHESIS OF DNA ENCODING THE AMINO TERMINUS 1. Synthesize a double - stranded DNA adapter extending from ...
Page 17-25
... fragment prepared by one of the methods described above ( see pages 17.18-17.24 ) . In most cases , a blunt - ended fragment will be inserted and this step will not be required . In a few cases , the gene fragment prepared as described ...
... fragment prepared by one of the methods described above ( see pages 17.18-17.24 ) . In most cases , a blunt - ended fragment will be inserted and this step will not be required . In a few cases , the gene fragment prepared as described ...
Page 1-12
... fragment ( large fragment ) , reac- tions and uses , 5.34 , 5.40-5.43 DNA sequence analysis by dideoxy- mediated chain - termination meth- od , 13.7-13.8 , 13.59-13.64 , 13.73- 13.74 labeling of synthetic oligonucleotides , 11.40-11.44 ...
... fragment ( large fragment ) , reac- tions and uses , 5.34 , 5.40-5.43 DNA sequence analysis by dideoxy- mediated chain - termination meth- od , 13.7-13.8 , 13.59-13.64 , 13.73- 13.74 labeling of synthetic oligonucleotides , 11.40-11.44 ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml