Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 1628
... gene and express moderate amounts of the protein of interest . Larger quantities of foreign protein can be obtained from lines of cells carrying amplified chromosomal copies of the gene of interest . In this method , the foreign gene is ...
... gene and express moderate amounts of the protein of interest . Larger quantities of foreign protein can be obtained from lines of cells carrying amplified chromosomal copies of the gene of interest . In this method , the foreign gene is ...
Page 1671
... gene . The above three examples illustrate the type of selection or screening that is essential for the initial identification of cells that have taken up and express the desired gene . Several strategies have been used to actually ...
... gene . The above three examples illustrate the type of selection or screening that is essential for the initial identification of cells that have taken up and express the desired gene . Several strategies have been used to actually ...
Page 17-15
... gene 1 and can be provided on an infecting bacteriophage A vector or produced from a gene copy inserted into the E. coli chromosome ( Tabor and Richardson 1985 ; Studier and Moffatt 1986 ) . If expression of the cloned gene product is ...
... gene 1 and can be provided on an infecting bacteriophage A vector or produced from a gene copy inserted into the E. coli chromosome ( Tabor and Richardson 1985 ; Studier and Moffatt 1986 ) . If expression of the cloned gene product is ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml