Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 1635
... growth medium and the time of treatment are limited by the sensitivity of the cells to the toxic effect of the drug . The optimal concentration of chloroquine for the particular cell type that is used should therefore be determined in ...
... growth medium and the time of treatment are limited by the sensitivity of the cells to the toxic effect of the drug . The optimal concentration of chloroquine for the particular cell type that is used should therefore be determined in ...
Page A-9
... growth of phagemids . It makes high levels of lac repressor and is used for inducible expression of genes that are under the control of the lac promoter . This strain can be used for detection of recombinants expressing B ...
... growth of phagemids . It makes high levels of lac repressor and is used for inducible expression of genes that are under the control of the lac promoter . This strain can be used for detection of recombinants expressing B ...
Page A-10
... growth of vectors carrying amber mutations and will modify but not restrict transfected DNA ( Yanisch - Perron et al . 1985 ) . A suppressing strain used to propagate bacteriophage vectors and their recombinants ( Wood 1966 ) . A strain ...
... growth of vectors carrying amber mutations and will modify but not restrict transfected DNA ( Yanisch - Perron et al . 1985 ) . A suppressing strain used to propagate bacteriophage vectors and their recombinants ( Wood 1966 ) . A strain ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml