Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 61
Page B-15
... hybridization Benton / Davis hybridization all Southern hybridizations other than single - copy dot blots 1 × BLOTTO ( Bovine Lacto Transfer Technique Optimizer ; Johnson et al . 1984 ) is 5 % nonfat dried milk dissolved in water ...
... hybridization Benton / Davis hybridization all Southern hybridizations other than single - copy dot blots 1 × BLOTTO ( Bovine Lacto Transfer Technique Optimizer ; Johnson et al . 1984 ) is 5 % nonfat dried milk dissolved in water ...
Page 1-18
... hybridization probes , 11.52-11.53 labeling , 11.15-11.16 3H , isotopic data , B.8 H HAT ( hypoxanthine , aminopterin , and thymidine ) medium , use as selec- tive agent , 16.9 , 16.14 HB101 , E. coli strain genotype and uses , A.10 ...
... hybridization probes , 11.52-11.53 labeling , 11.15-11.16 3H , isotopic data , B.8 H HAT ( hypoxanthine , aminopterin , and thymidine ) medium , use as selec- tive agent , 16.9 , 16.14 HB101 , E. coli strain genotype and uses , A.10 ...
Page 1-29
... hybridization and autoradiography , 7.52 staining RNA , 7.42 , 7.44-7.45 , 7.51 transfer of RNA to solid supports ... Hybridization activated , for western blotting , 18.64 capillary transfer of nucleic acids to supports , 7.49-7.50 ...
... hybridization and autoradiography , 7.52 staining RNA , 7.42 , 7.44-7.45 , 7.51 transfer of RNA to solid supports ... Hybridization activated , for western blotting , 18.64 capillary transfer of nucleic acids to supports , 7.49-7.50 ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml