Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 33
Page 18-42
... immunoglobulin antibody that reacts with species - specific determinants displayed on the antibody that interacts with the target antigen . This method is used under the following condi- tions : • When the Fc portion of the primary ...
... immunoglobulin antibody that reacts with species - specific determinants displayed on the antibody that interacts with the target antigen . This method is used under the following condi- tions : • When the Fc portion of the primary ...
Page 18-43
... immunoglobulins in serum is approximately 10 mg / ml . Super- natants from cultured hybridoma cells typically contain 10-100 μg / ml immuno- globulin , and ascites fluid contains 1-20 mg / ml immunoglobulin . 2. Incubate the mixtures ...
... immunoglobulins in serum is approximately 10 mg / ml . Super- natants from cultured hybridoma cells typically contain 10-100 μg / ml immuno- globulin , and ascites fluid contains 1-20 mg / ml immunoglobulin . 2. Incubate the mixtures ...
Page 18-45
... immunoglobulin , and ascites fluid contains 1-20 mg / ml immunoglobulin . 4. Collect the tertiary protein A - antigen - antibody complexes by centrifuga- tion at 12,000g for 20 seconds at 4 ° C in a microfuge . Remove the supernatant by ...
... immunoglobulin , and ascites fluid contains 1-20 mg / ml immunoglobulin . 4. Collect the tertiary protein A - antigen - antibody complexes by centrifuga- tion at 12,000g for 20 seconds at 4 ° C in a microfuge . Remove the supernatant by ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml