Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 1618
... levels of expression in a wide variety of transfected mammalian cells and high levels of expression in transfected COS cells • Both genomic DNA and cDNA sequences can be expressed , and there are no constraints on the size of the ...
... levels of expression in a wide variety of transfected mammalian cells and high levels of expression in transfected COS cells • Both genomic DNA and cDNA sequences can be expressed , and there are no constraints on the size of the ...
Page 172
... levels of protein are produced , additional steps may be taken to increase protein production ; if high levels of protein are produced , purification is relatively easy . Often , proteins expressed at high levels are found in insoluble ...
... levels of protein are produced , additional steps may be taken to increase protein production ; if high levels of protein are produced , purification is relatively easy . Often , proteins expressed at high levels are found in insoluble ...
Page 17-34
... level of protein produced . However , if the protein is made in an inactive form or is sequestered in inclusion bodies , then activity may not parallel the level of expression . In some cases , the goal is to make high levels of protein ...
... level of protein produced . However , if the protein is made in an inactive form or is sequestered in inclusion bodies , then activity may not parallel the level of expression . In some cases , the goal is to make high levels of protein ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml