Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 1648
... mammalian cells by fusing protoplasts , prepared from bacteria carrying the plasmid DNA of interest , with cultured cells ( Schaffner 1980 ; Rassoulzadegan et al . 1982 ) . The bacteria are grown in the presence of chloramphenicol to ...
... mammalian cells by fusing protoplasts , prepared from bacteria carrying the plasmid DNA of interest , with cultured cells ( Schaffner 1980 ; Rassoulzadegan et al . 1982 ) . The bacteria are grown in the presence of chloramphenicol to ...
Page 1652
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Fusion of Protoplasts to Mammalian Cells Growing in Suspension 1. Recover the mammalian cells growing in suspension by centrifugation at 1000g for 5 minutes at 4 ° C . Wash the cells ...
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Fusion of Protoplasts to Mammalian Cells Growing in Suspension 1. Recover the mammalian cells growing in suspension by centrifugation at 1000g for 5 minutes at 4 ° C . Wash the cells ...
Page 1657
... mammalian promoters . However , not all of these are suitable for use in transient expression systems . For example , the bacterial gpt ( Mulligan and Berg ... cells with unlabeled Expression of Cloned Genes in Cultured Mammalian Cells 16.57.
... mammalian promoters . However , not all of these are suitable for use in transient expression systems . For example , the bacterial gpt ( Mulligan and Berg ... cells with unlabeled Expression of Cloned Genes in Cultured Mammalian Cells 16.57.
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml