Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 1635
... medium for the isolation of stable transformants . b . In many instances , uptake of DNA is increased by concurrent treat- ment of the cells with chloroquine , which may act by inhibiting the degradation of the DNA by lysosomal ...
... medium for the isolation of stable transformants . b . In many instances , uptake of DNA is increased by concurrent treat- ment of the cells with chloroquine , which may act by inhibiting the degradation of the DNA by lysosomal ...
Page 1647
... medium ( GIBCO ) containing 10 % fetal calf serum . Incubate the cultures for 18-20 hours at 37 ° C in a humidified incubator in an atmo- sphere of 5-7 % CO2 . 2. Replace the medium with 3 ml of serum - containing medium containing DNA ...
... medium ( GIBCO ) containing 10 % fetal calf serum . Incubate the cultures for 18-20 hours at 37 ° C in a humidified incubator in an atmo- sphere of 5-7 % CO2 . 2. Replace the medium with 3 ml of serum - containing medium containing DNA ...
Page 18-28
... medium to the Med AA used for radiolabeling . Some types of cultured cells deteriorate rapidly when incubated in the absence of serum . In this case , it may be necessary to supplement the medium used for radiolabeling with serum that ...
... medium to the Med AA used for radiolabeling . Some types of cultured cells deteriorate rapidly when incubated in the absence of serum . In this case , it may be necessary to supplement the medium used for radiolabeling with serum that ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml