Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 1631
... method is simple and appears to be efficient , it is comparatively new and untested ( but see Felgner and Holm 1989 ; Maurer 1989 ) . • Direct microinjection into nuclei ( Capecchi 1980 ) . Although this method has the advantage of not ...
... method is simple and appears to be efficient , it is comparatively new and untested ( but see Felgner and Holm 1989 ; Maurer 1989 ) . • Direct microinjection into nuclei ( Capecchi 1980 ) . Although this method has the advantage of not ...
Page E-6
... METHOD Contaminants that may be present in the DNA sample can either contribute to or quench the fluorescence . To avoid these problems , the DNA samples and standards can be spotted ... METHOD E 6 AGAROSE PLATE METHOD E 6 MINIGEL METHOD E 6.
... METHOD Contaminants that may be present in the DNA sample can either contribute to or quench the fluorescence . To avoid these problems , the DNA samples and standards can be spotted ... METHOD E 6 AGAROSE PLATE METHOD E 6 MINIGEL METHOD E 6.
Page 1-6
... method , 16.37 cells growing in suspension , method , 16.38 modified ( Chen and Okayama ) method , 16.39-16.40 Calf intestinal alkaline phosphatase . See CIP Capillary transfer of nucleic acids from agarose gels to solid supports . See ...
... method , 16.37 cells growing in suspension , method , 16.38 modified ( Chen and Okayama ) method , 16.39-16.40 Calf intestinal alkaline phosphatase . See CIP Capillary transfer of nucleic acids from agarose gels to solid supports . See ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml