Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 18-35
... microfuge tube . This is best done with a red - hot hypodermic needle ( 23 gauge ) . Place the microfuge tube containing the glass beads inside an empty microfuge tube . Centrifuge the piggybacked pair of tubes in a 15 - ml Corex tube ...
... microfuge tube . This is best done with a red - hot hypodermic needle ( 23 gauge ) . Place the microfuge tube containing the glass beads inside an empty microfuge tube . Centrifuge the piggybacked pair of tubes in a 15 - ml Corex tube ...
Page 18-38
... microfuge tube . This prevents the glass beads from exploding out of the tube during heating . 8. Heat the tube in a boiling - water bath for 3 minutes . 9. Transfer the glass beads into a fresh microfuge tube , and wash the original tube ...
... microfuge tube . This prevents the glass beads from exploding out of the tube during heating . 8. Heat the tube in a boiling - water bath for 3 minutes . 9. Transfer the glass beads into a fresh microfuge tube , and wash the original tube ...
Page 18-39
... microfuge tube containing the glass beads inside an empty microfuge tube . Centrifuge the piggybacked pair of tubes in a 15 - ml Corex tube at 2000g for 2 minutes at 4 ° C . 11. Recover the bottom microfuge tube , which should contain ...
... microfuge tube containing the glass beads inside an empty microfuge tube . Centrifuge the piggybacked pair of tubes in a 15 - ml Corex tube at 2000g for 2 minutes at 4 ° C . 11. Recover the bottom microfuge tube , which should contain ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml