Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 1606
... nucleotides , AAUAAA , located 11-30 nucleotides upstream , which is necessary but not sufficient for posttranscriptional cleavage and polyadenylation ( for review , see Mason et al . 1986 ; Proudfoot and Whitelaw 1988 ) . The practical ...
... nucleotides , AAUAAA , located 11-30 nucleotides upstream , which is necessary but not sufficient for posttranscriptional cleavage and polyadenylation ( for review , see Mason et al . 1986 ; Proudfoot and Whitelaw 1988 ) . The practical ...
Page 1607
... nucleotides . Following polyadenylation of the primary transcript , the introns are removed by splicing to generate the mature mRNA , which is then transported from the nucleus to the cytoplasm ( for review , see Nevins 1983 ; Green ...
... nucleotides . Following polyadenylation of the primary transcript , the introns are removed by splicing to generate the mature mRNA , which is then transported from the nucleus to the cytoplasm ( for review , see Nevins 1983 ; Green ...
Page 1-12
... nucleotides , 15.108 generation of blunt - ended cDNA , 8.23 nick translation of DNA , 10.6-10.12 second - strand cDNA synthesis , 8.14- 8.20 , 8.64 Klenow fragment ( large fragment ) , reac- tions and uses , 5.34 , 5.40-5.43 DNA ...
... nucleotides , 15.108 generation of blunt - ended cDNA , 8.23 nick translation of DNA , 10.6-10.12 second - strand cDNA synthesis , 8.14- 8.20 , 8.64 Klenow fragment ( large fragment ) , reac- tions and uses , 5.34 , 5.40-5.43 DNA ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml