Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 1663
... phase to a fresh tube . Add 100 μl of ice - cold ethyl acetate to the original tube ( the aqueous phase ) . Vortex vigorously and centrifuge again as in step 3. Transfer 100 μl of the organic phase to the tube containing 80 μl of ethyl ...
... phase to a fresh tube . Add 100 μl of ice - cold ethyl acetate to the original tube ( the aqueous phase ) . Vortex vigorously and centrifuge again as in step 3. Transfer 100 μl of the organic phase to the tube containing 80 μl of ethyl ...
Page B-4
... phase . 2. To the melted phenol , add an equal volume of buffer ( usually 0.5 M Tris · Cl [ pH 8.0 ] at room temperature ) . Stir the mixture on a magnetic stirrer for 15 minutes , and then turn off the stirrer . When the two phases ...
... phase . 2. To the melted phenol , add an equal volume of buffer ( usually 0.5 M Tris · Cl [ pH 8.0 ] at room temperature ) . Stir the mixture on a magnetic stirrer for 15 minutes , and then turn off the stirrer . When the two phases ...
Page E-3
... phase forms the upper phase . However , if the aqueous phase is dense because of salt ( > 0.5 M ) or sucrose ( > 10 % ) , it will form the lower phase . The organic phase is easily identifiable because of the yellow color contributed by ...
... phase forms the upper phase . However , if the aqueous phase is dense because of salt ( > 0.5 M ) or sucrose ( > 10 % ) , it will form the lower phase . The organic phase is easily identifiable because of the yellow color contributed by ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml