Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 1571
... Plasmids 1.3 Replication and Incompatibility 1.3 Mobilization 1.5 Selectable Markers 1.5 Plasmid Vectors 1.7 DEVELOPMENT OF PLASMID CLONING VECTORS 1.7 Plasmid Vectors That Permit Histochemical Identification of Recombinant Clones 1.8 ...
... Plasmids 1.3 Replication and Incompatibility 1.3 Mobilization 1.5 Selectable Markers 1.5 Plasmid Vectors 1.7 DEVELOPMENT OF PLASMID CLONING VECTORS 1.7 Plasmid Vectors That Permit Histochemical Identification of Recombinant Clones 1.8 ...
Page 1572
... Plasmid DNA 1.31 Rapid Disruption of Bacterial Colonies to Test the Size of Plasmids 1.32 LARGE - SCALE PREPARATIONS OF PLASMID DNA 1.33 Amplification of Plasmids in Rich Medium 1.33 Harvesting and Lysis of Bacteria 1.34 HARVESTING 1.34 ...
... Plasmid DNA 1.31 Rapid Disruption of Bacterial Colonies to Test the Size of Plasmids 1.32 LARGE - SCALE PREPARATIONS OF PLASMID DNA 1.33 Amplification of Plasmids in Rich Medium 1.33 Harvesting and Lysis of Bacteria 1.34 HARVESTING 1.34 ...
Page 1-32
... Plasmid amplification by chloramphenicol , 1.3 , 1.21 , 1.23 , 1.33 cloning of DNA in plasmid vectors , 1.53 availability of appropriate restriction sites , 1.59 dephosphorylation of linear plasmid DNA , 1.60-1.62 determining extent of ...
... Plasmid amplification by chloramphenicol , 1.3 , 1.21 , 1.23 , 1.33 cloning of DNA in plasmid vectors , 1.53 availability of appropriate restriction sites , 1.59 dephosphorylation of linear plasmid DNA , 1.60-1.62 determining extent of ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml