Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 1598
... Precipitation with Ethanol or Isopropanol E.10 PRECIPITATION OF DNA IN MICROFUGE TUBES E.12 PRECIPITATION OF RNA WITH ETHANOL E.15 PRECIPITATION OF LARGE RNAS WITH LITHIUM CHLORIDE E.15 CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH ...
... Precipitation with Ethanol or Isopropanol E.10 PRECIPITATION OF DNA IN MICROFUGE TUBES E.12 PRECIPITATION OF RNA WITH ETHANOL E.15 PRECIPITATION OF LARGE RNAS WITH LITHIUM CHLORIDE E.15 CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH ...
Page E-10
A Laboratory Manual Joseph Sambrook, Tom Maniatis. CONCENTRATING NUCLEIC ACIDS Precipitation with Ethanol or Isopropanol The most widely used method for concentrating nucleic acids is precipitation with ethanol . The precipitate of ...
A Laboratory Manual Joseph Sambrook, Tom Maniatis. CONCENTRATING NUCLEIC ACIDS Precipitation with Ethanol or Isopropanol The most widely used method for concentrating nucleic acids is precipitation with ethanol . The precipitate of ...
Page E-14
... precipitation with ethanol . The second precipitation may help eliminate additional salts or other components that may be preventing dissolution of the DNA . PRECIPITATION OF RNA WITH ETHANOL RNA is efficiently precipitated with E.14 ...
... precipitation with ethanol . The second precipitation may help eliminate additional salts or other components that may be preventing dissolution of the DNA . PRECIPITATION OF RNA WITH ETHANOL RNA is efficiently precipitated with E.14 ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml