Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 1593
... Production of Fusion Proteins 17.3 Vector Systems for the Expression of lacZ Fusion Genes 17.4 Construction of Expression Plasmids and Detection of Fusion Proteins 17.8 Preparation of Fusion Proteins for Antibody Production Production ...
... Production of Fusion Proteins 17.3 Vector Systems for the Expression of lacZ Fusion Genes 17.4 Construction of Expression Plasmids and Detection of Fusion Proteins 17.8 Preparation of Fusion Proteins for Antibody Production Production ...
Page 173
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Production of Fusion Proteins If antibodies to the protein encoded by the cloned DNA are not available , it is often useful to produce a fusion protein for use as an antigen . Gene ...
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Production of Fusion Proteins If antibodies to the protein encoded by the cloned DNA are not available , it is often useful to produce a fusion protein for use as an antigen . Gene ...
Page 17-9
... Production The fusion protein can be prepared for injection in a number of ways , including urea extraction of ... Production of Intact Native Proteins Intact native proteins can be Expression of Cloned Genes in Escherichia coli 17.9 ...
... Production The fusion protein can be prepared for injection in a number of ways , including urea extraction of ... Production of Intact Native Proteins Intact native proteins can be Expression of Cloned Genes in Escherichia coli 17.9 ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml